The bulletin of the Yamaguchi Medical School
Continued by：Medical Science & Innovation
EISSN：2758-5441
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The bulletin of the Yamaguchi Medical School Volume 10 Issue 3
published_at 1963-09
Establishment of MIN-Lymphosarcoma as a Permanent Cell Strain in Tissue Culture
Establishment of MIN-Lymphosarcoma as a Permanent Cell Strain in Tissue Culture
Mori Shigeki
Harada Yoshio
Yamaoka Ikuo
About This Article
Descriptions
Three tissue culture strains were established from MN-lymphosarcoma cells originally carried intraperitoneally in NA2 female mice. They have been maintained by serial subcultivation for more than 16 months. There were no apparent differences except for cell size in morphological features among the 3 trains. When inoculated into mice intermittetly after prolonged maintenance in vistro, strain No. 1 and No. 3 cells induced ascites tumor formation and a mortality of 80～100%. Strain No. 2 cells seemed to have lost their transplantability. When the cultured stain No. 1 cells were longer in the first passage, was shortened within a few geberations to that of tha original mouse-to-mouse passage. This result might be discussed on the hupothesis of a gradual transformaion of cells for adaptaion to growth in altered enviroment.
Other Article
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