Bulletin of the Faculty of Agriculture, Yamaguchi University

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Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 25
published_at 1974

Tissue culture of Frog's organs and Isolation of Japanese B encephalitis Virus

カエル臓器の組織培養と日本脳炎ウイルスの分離
Toda Mitsuyoshi
Kanoe Masamitsu
Mannami Saburo
Oota Hiroaki
Takayama Hiroo
Sato Akio
About This Article
Descriptions
日本脳炎ウイルスの越冬問題を究明するのを最終目的として，今回は野外で捕獲したカエルからのウイルス分離を主目的として実験をおこなった｡その結果を要約すると次のとおりである｡1.カエル臓器より直接日本脳炎ウイルスを分離することはできなかったが，カエルの腎臓細胞の組織培養を実施中ウイルスを分離した｡同定の結果日本脳炎ウイルスであることが判明した｡2.日本脳炎ウイルスを分離したカエルはトノサマガエルであり，1973年6月山口大学周辺で捕獲したものである｡3.カエル臓器の良効な組織培養法を開発するのは今後課題である｡しかしながら今回われわれがおこなったトリプシン消化をせずに細切，磨砕した組織による培養法で培養が可能であり，実験に供することができた｡しかも腎臓培養細胞で日本ウイルスが増殖することが判明した｡(昭和49年6月30日受理)
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