Bulletin of the Faculty of Agriculture, Yamaguchi University

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Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 16
published_at 1965

Studies on the Ribonuclease in Posterior Silkglands of Silkworm Larvae, Bombyx mori. : 2. Specincity of the Ribonuclease

家蚕後部絹糸腺のリボヌクレアーゼに関する研究 : 第Ⅱ報酵素の特異性
Numa Sinji
Mukai Jun-Itiro
Akune Satoru
About This Article
Descriptions
One of the two ribonucleases detected in posterior silkglands of silkworm was purified by using of ammonium sulfate fractionation, dialysis, carboxymethyl cellulose column chromatography and Sephadex G-100 gel filtration. The specificity was determined on the completely and incompletely digested yeast RNA by paper chromatography and column chromatography. This enzyme splits C'_5-phosphate bond of 3', 5'-phosphodiester linkages of ribonucleic acid. 2'-and 3'-Adenylic acid, guanylic acid, 3'-(and, or 2'-) cytidylie acid and uridylic acid (perhaps 3'-and, or 2'-) were formed, with cyclic mononucleotides as intermediate priduct.
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