Studies on Nonsporebearing Obligate Anaerobic Bacteria or Animals : I. Cultural Medium

Recently, the importance of nonsporebearing obligate anaerobic bacteria has been noticed, concerning with many kinds of diseases and nutritional failures both men and animals in many countries. While, it is well known that many anaerobes present in men and animals can not be isolated unless special prosedures are used. Considerable difficulties, consequently, exist to study these anaerobes in common laboratories at present. Therefore, as one of the basic studies on these problems, present work was undertaken to inbestigate medium for the isolation of anaerobes present in various sources. Comp rative studies used were as follows: The employed media were (1) thioglycollate agar, (2) cooked meat agar, (3) blood agar, (4) Zeissler agar, (5) reinforced clostridial medium, (6) Eggerth and Gagnon medium, (7) glucose yeast blood liver agar and (8) liver infusion agar. Using strains in this experiment were isolated from several animals. Fresh cultures of these strains were streaked on the surgace of hte each plates, and incubated with the method of phosphorous jar. Samples for bacteriological examination were collected from the feces of a pig, dogs, rabbits and the caecum of rabbits sacrificed. A one gram portion of the contents of each sample was weighed, and added to 9 ml of sterile saline solution in a test tube. 0.1 ml each of suitable diluted samples was spread on these plates with conradirods and incubated. The results obtained are summarized as follows: 1) Among the eight kinds of media used, liver infusion agar plate was the most excellent for the kinds, members, and growth of intestinal nonsporabearing obligate anaerobic bacteria. 2) It was evident that thioglycollate medium was not suitable for the isolation of nonsporebearing obligate anaerobic bacteria from various sources. 3) In view of the above mentioned facts and in respect of practical use, it was felt thet the use of liver infusion agar medium was the most available.