Studies on Clostridium welchii I. Cultural Method and Distributions in Pig Feces and Soil

Reccently, the importance of Clostridium uelchii have been noticed in connection with both alimentary disorders of domestic animals and food hygiene. Little consideration, however, was paid to the ecology with this microbe in healthy animals. Therefore, as one of the basic studies on these problems, the present work was undertaken to investigate methods for the culturing of Clostridium welchii, and to examine its distribution in the intestine of pigs and in the soil. Following results were obtained. 1). Method, culturing sample in Nagler medium after heated at 60℃ 10 minutes, was obtained good results for the isolation and enumeration of the bacterial counts with Clostridium welchii. 2). In many samples of pig feces examined, both bacterial counts of non heat resistant and heat resistant Clostridium welchii, were found at levels of 10^3-10^5/g and 10^2-10^4/g. 3). In many samples of soil examined, both bacterial counts of non heat resistant and heat resistant Clostridium welchii, were found at levels of 10^2-10-3/g and 10^1/g.