Studies on the microorganisms isolated from grass silage and cattele : 2. Lactic acid becteria isoland from Ladino clover silage and intestines of milch cows having fed down the silage

Cultured Lactobacillus plantarum and Lactobacillus brevis were added in Ladino clover silages. After 6 months, these silages were opened and evaluated. Twenty three strains of lactic acid bacteria were isolated from silages and intestines of milch cows having been fed on these silages, and then were identified. Since, in the silages being not only added above-mentioned lactobacilli but not added, Lactobacillus arabinosus was foumd in large numbers, it was thought that Lactobacillus arabinosus originated in Ladino clover. A number of Lactobacillus plantarum was also found in the silages being added this cultured bacteria, but on the contrary Lactobacillus brevis was found a little in the silage with the same treatment. It was thought as a reason that since pH of Ladino clover silages were somewhat higher than that of Italian rye-grass silages, Lactobacillus plantarum and Lactobacillus arabinosus having a weak acid tokerance, vigorously grew in clover silage rather than Lactobacillus brevis having a strong acid tolerance. As the other lactobacilli, Lactobacillus casei and Bacillus coagulans were isolated from the silages, but it was not thought that these bacteria served for main fermentation. Enterolactobacilli were isolated from just dropped dungs of cows and the results were as following