Bulletin of the Faculty of Agriculture, Yamaguchi University

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Bulletin of the Faculty of Agriculture, Yamaguchi University Volume 20
published_at 1969

Studied on Carotenoid pigments and Colour of Fruits in Japan : 1. A Determination Method of Carotenoids in Fruits

本邦産果実のカロチノイド色素と色調とに関する研究 : 第1報 果実中のカロチノイド定量法について
Kobayashi Kunihiko
Anno Takahiko
Akuta Saburo
Descriptions
A convenient method for chromatographic determination of carotenoids in fruits was studied. The carotenoids was separated employing column chromatography on magnesium oxide : Hyflo Super-Cel (1 to 1, w./w.). The column was developed with 5 and 10% aceton in n-hexan. In case of yellow peaches in Japan, ten fractions of carotenoids were obtained by single operation of chromatography. The major carotenoid in each fractions was found as follow