Recently, Shibata and Nozaka had reported the anthocyanin constitution of Gladiolus. As their results seemed to contain some unusual features from genetic point of view, the present re-investigation was conducted, in which some considerable amount of discrepancy was disclosed. The results obtained were summarized as follows: 1 Thirteen anthocyanins were identified. They were pelargonidin-3-rhamnosylglucoside, pelargonidin-3,5-doglucoside, pelargonidin-3-rhamnosylglucoside-5-glucoside, peonidin-3-rhamnosylglucoside, peonidin-3,5-diglucoside, peonidin-3-rhamnosylglucoside-5-glucoside, cyanidin-3,5-diglucoside, malvidin-3-rhamnosylgucoside, malvidin-3,5-diglucoside, malvidin-3-rhamnosylglucoside-5-glucoside, petunidin-3,5-diglucoside, petunidin-3-rhamnosylglucoside-5-glucoside and delphinidin-3,5-diglucoside.....