Studies on the Isolation of Virus from the So-called Canie Distemper in Tissue Culture : II. A case Report of Infectious Hepatitis of Dogs Virus Isolation

The authors treid to ioslate the virus from dogs clinically diagnosed as having canine distenper or analogous disease, through use of puppies kidney tissue culture. Rarely, isolated viral agents from puppies kidney cell culture. Several results of the experiments were shown as follows. 1. The authors isolated seven viral agents from 12 puppies kidney cell culture (contained a case of lung or spleen cell). This puppies was breed from same womb with 3 times breeding (almost used within 2 months). 2. TCID_50 of the fluid phase, at 7th generatiom were in the range of 10^<-5.8> -10^<-5.3>. Isolated viruses neutralized in the neutralization test. The neutralization test, in tissue culture, proved to be the most useful and sensitive methods for the diagnosis. 3. The studies on the complement-fixing antigen, the hemagglutinating faculty and the infective titer with virus in vitro (inicial inoculum, 2000 to 6000 TCID_<50>) indicated that the infective titer increased on the 2nd day accompanied by the inclusion bodies, reached the maximum titer on the 6th day after virus inoculation, and after that, remained at the same level, On the other hand, the complement-fixing antigen and hemagglutinating faculty was first detected on the about 3rd day after inoculation, when a cytopathogenic effect appeared. And then the complement-fixing antigen titer and hemagglutinating titer increased continuously reching 1 : 16 or 1 : 8 on the 6 th or 7th day after the virus inoculation. 4. The effects of various temperatures on the virus were examined. It was found that the infectivity of the virus was destroyed by heating of 56℃ for about 60 minutes or 65℃ for 2 minutes. At 37℃ this viruses were inactivated until 30th day after inoculation, respectively. The virus was infective to tissue culture when held at the -20℃ state for 1 years or at 4 ℃ for 6 months. At room temperature the virus kept its infectivity of 10 weeks. 5. Stability of the virus at 37℃ was examined. The results indicated that the complement-fixing antigen, hemagglutinating faculty and its infectivity were stable, in turn, at this temperature.