Separation and Determination of Phenothiazines 2. on the Method for Spectrophotometry

The methods for the spectrophotometric determination of six kinds of phenothiazines with both ferric chloride and arsenic acid regents. The realts were as follows. 1. Phenothiazines were allowed to react with ferric chloride reagent in Clark-Lub's buffer solution and the oxidized products thus formed were submitted to the measurement of visible spectrophotometry. The spectra derived from alimemazine, spichromazine, thioridazine and promethazine had the absorption maxima, but the spectra in chlorpromazine and trifluoperazine were somewhat similar to that of reagent blank and had not the maxima. In addition, the sensitivity of developed colors was very week and so, their calibration curves were not depicted…