Hematopoietic Tissue Allograft in Diffusion Chamber in Mice 2. Host Cell Reaction in Lymph Node Allograft

The host and graft cell interactions were observed following of transplantation of allogeneic lymph nodes in diffusion chamber. Most of animals were the same as used in the previous report. The mice, which were transplanted allogeneic mesenteric lymph nodes in diffusion chamber into their peritoneal cavities, were sacrificed at intervals of 1, 2, 3, 5, 7, 10, 15, 21, 30 and 60 days after transplantation. Thereafter, the chambers and the host thymolymphatic tissues were removed and examined microscopically. As control, the empty chamber bearing mice of the same age and sex were used. The cell-impenetrable filters (HA type) did not permit host cells to pass through them. The host cells were always seen throughout the thickness of type AA millipore filters and inside the chambers constructed of these filters. The first host cell to enter the filters were polymorphonunclear leukocytes. There were followed by lymphocytes and macrophages (Figs. 1-8).