Hematopoietic Tissue Allograft in Diffusion Chamber in Mice 1. Fate of Lymph Node and Bone Marrow Allograft in Diffusion Chamber

Using the diffusion chamber, survival of lymph node and bone marrow allograft was investigated. Mice used as donors and hosts were 2-month-old mice of randomly bred ddT and ddY strains and of inbred strains Cb, Strong A, Db and C_57BL. Pieces of the mesenteric lymph nodes or femoral bone marrows obtained from male and female donor mice were placed in the diffusion chambers, then were implanted into the peritoneal cavities of the allogeneic host mice of the comparative sex. The host mice were killed at intervals of 15, 21, 30 and 60 days following implantation. The chambers employed were a modification of that of Algire et al (1954, 1955). The construction of the chamber and histologic examination of their contents were described in detail elsewhere (Awaya, 1964). Many of the lymph node allografts in cell-impenetrable diffusion chamber (constructed of HA type millipore filters, pore size 0.45μ) survived from 15 to 60 days after implantation, although some of them were injured in varying degrees (Fig.1-12