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Histochemical study of otoconial formation in chick embryos

山口医学 Volume 40 Issue 2 Page 171-179
published_at 1991-04
Title
鶏胚耳石の組織化学的研究 : 耳石発生における複合糖質の局在について
Histochemical study of otoconial formation in chick embryos
Creators Endo Shiro
Source Identifiers
Histochemical investigation was done to clarity the distributon of glyacocojugates in the otolithic organs of developing chick embryos. Two kinds of lectins, wheat germ agglutinin and concanacalin A, were appilied to detect the localization of N-acethyl-glucosaminyl and mannosyl residues. FITC- and TRITC-labelled lectins were applied in preparation for fluorescence microscopy and colloidal-gold labelled lectins for transmission electron microscopy. In the 6-day-old chick embryo, the globular and the granular lectin-positive substances, which are regarded as the precursors of the otoconia, were observed both on the surface of and within the sensory epithelium of the macula. The otoconia were intensely labelled with lectins at every developmental stage of the chick embryo, while the labelling on the macular sensory epithelium decreased as the otoconia matured. Transmission electron microscopic observation revealed the localization of the glycoconjugates on secretory gramules of suppoerting cells of the sensory erithelium. At every stage of the developing chick embryuos, the lectin labellings in the otoconia were more intense in the peripheral electron dense zones, which had a higher calcium content, than in the central electron lucent zone. It was concluded that the precursors of the otoconia were secreted from the supprting cells of the macular sensory epithelium, and that the glycoconjugates play an important role in otoconial formation, strongly relating to calcium deposition.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1991-04
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部