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A Simple and Rapid Colorimetric Determination for Nitrazepam in Alkaline Solution

山口医学 Volume 33 Issue 3 Page 157-166
published_at 1984-06
Title
アルカリ性溶液中でのニトラゼパムの簡便, 迅速化色定量法
A Simple and Rapid Colorimetric Determination for Nitrazepam in Alkaline Solution
Creators Sugawara Norisuke
Creators Nakayasu Kiyoshi
Creators Furuno Junji
Source Identifiers
Several investigations in our laboratory have shown that nitrazepam turns yellow in alkaline medium and that the color solution in the ultraviolet region has maximum absorption at a wavelength of 366 nm. In the study here, chromatic reactions were studied at 10^0, 10^<-1>, 10^<-2>, 10^<-3> and 10^<-4> N concentrations of sodium hydroxide. The most suitable result was obtained with 10^<-1> sodium hydroxide and the optical density was intensified by heating in a water bath at 100℃ for 30 minutes. The calibration curve was linear at 2 to 16 μg/ml concentration and its regression equation was given by Y=0.069X -0.017 (0.063<β<0.075, α=0.05), where Y is absorbance at 370 nm and X is the concentration of nitrazepam (μg/ml). This method of colorimetric determination for nitrazepam is simple, rapid and reproducible. Hence, it is considered applicable for analysis of nitrazepam in forensic chemistry.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1984-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部