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Studies on the Reversed Passive Latex Agglutination for the Detection of Enterotoxin : Part1. Detection of Cholera Toxin and Heat-Labile Enterotoxin of Escherichia coli

山口医学 Volume 32 Issue 3 Page 259-268
published_at 1983-06
Title
逆受身ラテックス凝集反応によるエンテロトキシン検出法の研究 : 第1報 コレラ毒素および毒素原性大腸菌易熱性毒素についての研究
Studies on the Reversed Passive Latex Agglutination for the Detection of Enterotoxin : Part1. Detection of Cholera Toxin and Heat-Labile Enterotoxin of Escherichia coli
Creators Sugiyama Junichi
Source Identifiers
Reversed passive latex agglutination (RPLA) for the detection of cholera toxin (CT) and heat-labile enterotoxin (LT) of Escherichia coli was studied. The assay was based on the immunological similarity between CT and LT. For the sensitization of latex, immunoglobulins against CT were purified by affinity chromatography. A suitable dose of immunoglobulin for sensitization of 0.5% latex suspension was the same amount of 25 μg/ml. It gave detectable agglutination reactions at a concentration of 1 ng/ml of CT. Good correspondence between the results obtaintd by the RPLA test and those by Y_1 adrenal cell assay was observed. The technical procedures of this method is very simple and easy. Also, the results are reliable. The sensitized latex is considered to be stable for at least 1 year when stored at 4℃. Therefore, this method would be recommended for the tests of CT and LT in bacteriology, clinical bacteriology as well as epidemiology of acute enteritis.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1983-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部