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Expression of MHC Antigents in Fetal Rat Thymus

The bulletin of the Yamaguchi Medical School Volume 35 Issue 3-4 Page 35-41
published_at 1988-12
A020035000301.pdf
[fulltext] 1.54 MB
Title
Expression of MHC Antigents in Fetal Rat Thymus
Creators Kuniki Hiromichi
Creators Fujikura Yoshihisa
Creators Fukumoto Tetsuo
Source Identifiers
Creator Keywords
HMC antigen fetal thymus Monoclonal antibodies Thymic epithelial cells
Major histocompatibility complex (MHC) Class I antigen is known distinct on cell surface of the mature cells including lymphocytes but not detected on early stage of thymocytes. Monoclonal antibody, OX-18 or HAM-2 is the specific antibody to rat MHC Class I antigen and OX-6 is that to MHC Class II. These antibodies are detected immunohistochemically or flow cytofluorometrically by using HRP or FITC conjugated anti-mouse immunoglobulin. Main findings are as follows : MHC Class I antigen first appeared on thymic epithelial cells at day 15 of gestation and with the progress of fetal stage MHC Class I antigen positive cells appeared to increase in number and its intensity of the staining. MHC Class I antigen on thymocytes at about day 15 of gestation was negative, however at day 19 of gestation was about 36%, which is same as in normal young adult thymus. The degree of total antigen amount expressed in fetal thymus was compared with that in adult thymus, using radioactive binding inhibition assay. The amount of antigen is quite low at days 18 and 20 of gestation compared to that of adult. From these findings it is suspected that MHC Class I antigen first appeared on epithelial cells and a few days later on thymocytes. Thus, the interaction between MHC Class I positive epithelial cells and thymocytes may be important for the differentiation and maturation of thymocytes.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1988-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部