Contents Menu

Staining of Proliferating Cell Nuclei with Biotinylated dUTP

The bulletin of the Yamaguchi Medical School Volume 38 Issue 3-4 Page 41-48
published_at 1991-12
A020038000301.pdf
[fulltext] 1.99 MB
Title
Staining of Proliferating Cell Nuclei with Biotinylated dUTP
Creators Murakami Tomoyuki
Source Identifiers
Creator Keywords
Biotinylated dUTP BrdU DNA systhesis Flow cytometry
A method of in situ biotinylated dUTP incorporation into nuclei was developed for evaluaton of cell growth activity. HeLa cells or frozen sections of cancer tissues were incubated in a solution containing bio-dUTP and the other deoxyribonucleotides (dATP, dCTP, dGTP), after permeabilization of cell membrane with Triton X-100, and signals from bio-dUTP in nuclei were detected with an avidin-biotin-peroxidase complex (ABC method), or with fluoresceinavidin. This staining procedure was simple and rapid as compared with that for BrdU incorporated into DNA. In HeLa cells, bio-dUTP-incorporated cells could be stained by the ABC method and the labeling index was compatible with that of BrdU, but the intranuclear distribution patterns of bio-dUTP-incorporated cells was also possible. In some frozen sections of cancer tissues, cells synthesizing DNA were labeled with bio-dUTP. These results suggest that this will be a useful method for evaluating cell growth activity, although the biological significance may be different the BrdU incorporation experiment.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1991-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部