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Separation Methods of Epitheloid and Fibroblastic Cells in Tissue Culturing of Peritoneal Exudates of Mink

The bulletin of the Yamaguchi Medical School Volume 19 Issue 1-2 Page 1-16
published_at 1972-06
A020019000101.pdf
[fulltext] 3.01 MB
Title
Separation Methods of Epitheloid and Fibroblastic Cells in Tissue Culturing of Peritoneal Exudates of Mink
Creators Yoshii Zensaku
Creators Padgett George A.
Creators Farrell Keith R.
Source Identifiers
Usefulness and Necessity of separation of epitheloid and fibroblastic cells in tissue sulturing were emphasized. Two methods with several techniques were examined by using mink cells from peritoneal exudates. Harvesting method of necessary cells or using mink cells from peritoneal exudates. Harvesting method of necessary cells or colonies contains three techniques. They are punching for both epitheloid and fibroblastic cells, slight digestion with ATV-solution for fibroblastic cells mainly, and scratching for fibroblastic cells. Elimmination method of unnecessary cells or colonies countains two techniques. They are erasing (almost same as scratching) and slight digestion. The methods with these tecniques have been proven practical for separation. Finally, a systematic figure of the technical procedures and methods was established by the authors. Selection of suitable flasks, adoption of adequate technique and method, combination of techniques, and repetition of techniques are recommended for routine work in the separation of one type cell. Efficiency and purity of the results are also discussed.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1972-06
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部