Separation Methods of Epitheloid and Fibroblastic Cells in Tissue Culturing of Peritoneal Exudates of Mink

Separation Methods of Epitheloid and Fibroblastic Cells in Tissue Culturing of Peritoneal Exudates of Mink

Creators Yoshii Zensaku

Creators Padgett George A.

Creators Farrell Keith R.

Usefulness and Necessity of separation of epitheloid and fibroblastic cells in tissue sulturing were emphasized. Two methods with several techniques were examined by using mink cells from peritoneal exudates. Harvesting method of necessary cells or using mink cells from peritoneal exudates. Harvesting method of necessary cells or colonies contains three techniques. They are punching for both epitheloid and fibroblastic cells, slight digestion with ATV-solution for fibroblastic cells mainly, and scratching for fibroblastic cells. Elimmination method of unnecessary cells or colonies countains two techniques. They are erasing (almost same as scratching) and slight digestion. The methods with these tecniques have been proven practical for separation. Finally, a systematic figure of the technical procedures and methods was established by the authors. Selection of suitable flasks, adoption of adequate technique and method, combination of techniques, and repetition of techniques are recommended for routine work in the separation of one type cell. Efficiency and purity of the results are also discussed.

[ISSN]0513-1812

[NCID]AA00594272