Detection of D-Xylose Isomerase on Disc Electrophoresis
The bulletin of the Yamaguchi Medical School Volume 18 Issue 1-2
Page 1-5
published_at 1971-06
Title
Detection of D-Xylose Isomerase on Disc Electrophoresis
Creators
Nakamura Shojiro
Creators
Shimizu Jiro
Creators
Yamanaka Kei
Source Identifiers
D-Xylose isomerase was crytallized from Lactocillus brevis cultered in a medium containing D-xylose. Protein fractions obtained at various steps of the purification were analyzed by disc electrophoresis. D-Xylose isomerase activity was detested in the disc gels utilizing the formation of formazan dye from triphenyltetrazolium chloeide by ketose. The crystalline preparation of the enzyme was pure on disc electrophoresis and found to catalyze the isomerization of other D-sugars: D-arabinose, D-ribose, D-glucose, D-mannose, and D-galactose. L-Arabinose was found tobe isomerized by another fraction than D-xylose isomerase.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1971-06
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部