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The Role of Extracellular Calcium on Zinc Hydroxide-Induced Superoxide Production in Rat Neutrophils

The bulletin of the Yamaguchi Medical School Volume 43 Issue 3-4 Page 83-89
published_at 1996-12
A020043000308.pdf
[fulltext] 649 KB
Title
The Role of Extracellular Calcium on Zinc Hydroxide-Induced Superoxide Production in Rat Neutrophils
Creators Egami Norio
Source Identifiers
Creator Keywords
Zinc hydroxide Superoxide Neutrophils Zinc fume fever
The purpose of this work was to study whether extracellular calcium concentration and voltage-gated calcium channels might influence zinc hydroxide-in-duced superoxide production in rat neutrophils. An increase in extracellular calcium concentration augmented superoxide production. Zinc hydroxide caused an elevation of intracellular free calcium concentration ([Ca2+]I) in parallel with extracellular calcium concentration. Four calcium channel antagonists were tested for their ability to inhibit superoxide production. The relative order of potency of antagonists was varapamil > diltiazem > nifedipine. Flunarizine was ineffective. The calmodulin antagonist (W-7)and the intracellar free calcium chalactor, 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA/AM), inhibited superoxide production. The inhibition of supperoxide production by a tyrosine kinase inhibitor, 4',5,7-trihydroxyisoflavone (genistein), was 50% of maximum. The results suggest that zinc hydroxide can stimulate superoxide production in neutrophils mainly by opening the voltage-gated calcium channels, facilitating the influx of extracellular calcium and an elevation of [Ca2+]I.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1996-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部