Effects of Endogenous Pyrogen and Prostaglandin E_2 on Preoptic and Anterior Hypothalamic Neurons in Slice Preparation of Guinea-pig

モルモットの視床下部神経細胞活動に対する内因性発熱物質とプロスタグランディンE_2の影響

Effects of Endogenous Pyrogen and Prostaglandin E_2 on Preoptic and Anterior Hypothalamic Neurons in Slice Preparation of Guinea-pig

Creators Ono Takuya

To investigate the effects of EP and PGE_2 on the activity of the neuron in the PO/AH, single unit activity was recorded from in vitro brain tissue slices prepared from the PO/AH of guinea-pigs. Of 94 PO/AH neurons studied, 81% of the neurons was warm-responsive neuron, 3% cold-responsive and 16% temperature-insensitive neurons. With an application of EP into perfusate, 41% of 73 warm-responsive neurons altered their activities. The same proportion of 51 warm-responsive neurons as the effects of EP application was also observed with PGE_2 administration. However, these changes in the firing rate varied depending upon their own thermosensitivities. When perfusate was substituted by low Ca^<2+>, high Mg^<2+> Krebs-Ringer solution, 40% of the temperature-responsive neurons lost their thermosensitivities. The temperature-responsive neurons remaining their thermosensitivities did not show any changes of response to EP or PGE_2 before, during and after synaptic blockade.

[ISSN]0513-1731

[NCID]AN00243156