Establishment of MIN-Lymphosarcoma as a Permanent Cell Strain in Tissue Culture

Establishment of MIN-Lymphosarcoma as a Permanent Cell Strain in Tissue Culture

Creators Mori Shigeki

Creators Harada Yoshio

Creators Yamaoka Ikuo

Three tissue culture strains were established from MN-lymphosarcoma cells originally carried intraperitoneally in NA2 female mice. They have been maintained by serial subcultivation for more than 16 months. There were no apparent differences except for cell size in morphological features among the 3 trains. When inoculated into mice intermittetly after prolonged maintenance in vistro, strain No. 1 and No. 3 cells induced ascites tumor formation and a mortality of 80～100%. Strain No. 2 cells seemed to have lost their transplantability. When the cultured stain No. 1 cells were longer in the first passage, was shortened within a few geberations to that of tha original mouse-to-mouse passage. This result might be discussed on the hupothesis of a gradual transformaion of cells for adaptaion to growth in altered enviroment.

[ISSN]0513-1812

[NCID]AA00594272