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Induction to Granulocytic Differentiation of Human Promyelocytic Leukemia Cells (HL-60) by Polyprenoic Acid (E5166)

The bulletin of the Yamaguchi Medical School Volume 33 Issue 1 Page 1-7
published_at 1986-12
A020033000101.pdf
[fulltext] 968 KB
Title
Induction to Granulocytic Differentiation of Human Promyelocytic Leukemia Cells (HL-60) by Polyprenoic Acid (E5166)
Creators Sasaki Kohsuke
Creators Murakami Tomoyuki
Creators Ogino Tetsuro
Creators Kawasaki Shoji
Creators Okita Kiwamu
Creators Takahashi Manabu
Source Identifiers
Creator Keywords
Differentitation Leukemia polyprenoic acid Flow cytometry BrdUrd Cell cycle
The effects of synthesized polyprenoic acid, E5166,on human promyelocytic leukemia cell line, HL60, were examined. The cells continuously exposed to the drug in vitro underwent growth inhibition and granulocytic differentation. In the culture treated with E5166, the cell cycle progression was markedly inhibited and an accumukation of the cells in G1/0 phase was apparent on day 4 when a small number of granulocyte first appeared. Subsequently, the percentage of differentiated cellsin the culture increased continuously with the time of treatment and on day 7, 80% of cells acquired the morphological characteristics of mature granulocytes. The close relationship between cell kinetics and differentiation was suggested, although the mechanisms by which E5166 induces differentiation and exerts its antiproliferative effect on HL-60 are unknown.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1986-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部