We inverstigated the appearance date of pupal diapause butterfly species in Yamaguchi City during spring from March to May, 2006. This survey recorded the appearance date of 16 pupal diapause butterfly species in Yamaguchi City, and identified 9 papilionids, 3 pierids, 2 satyrids, 1 nymphalid and 1 lycaenid. In the spring of 2006, Pieis rapae (Pieridae) was first to appear from March 7 around urban districts of Yamaguchi City, and then Papilio machaon and Pa. xuthus (Papilionidae) appeared within similar areas from late March. Anthocharis scolymus (Pieridae), Arachunia burejana (Nymphalidae) and Rapala arata (Lycaenidae) appeared from the middle of April around upland areas, and another 6 papilionids and 2 satyrids were observed from May in hill areas around Yamaguchi City. These data provide a benchmark of the appearance date fo pupal diapause butterfly species in Yamaguchi City, Japan.