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Tsuneoka Hidehiro

Affiliate Master Yamaguchi University

A Method Detecting Bacteria in Culture Medium by Simultaneous Measurement of Electrical Impedance and Turbidity

Memoirs of the Faculty of Engineering, Yamaguchi University Volume 30 Issue 2 Page 241-247
published_at 1980
KJ00000156301.pdf
[fulltext] 561 KB
Title
細菌の電気的・光学的同時測定による自動検査法(第 2 報)
A Method Detecting Bacteria in Culture Medium by Simultaneous Measurement of Electrical Impedance and Turbidity
Creators Hashimoto Hajime
Creators Miike Hidetoshi
Creators Ebina Yoshio
Creators Tsuneoka Hidehiro
Creators Miyaji Takaoki
Source Identifiers
An identification method is proposed for some bacteria by utilizing the parameters obtained from the simultaneous measurement of electrical impedance and turbidity. The examined bacteria are Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Serratia marcescens, Proteus morganii and Streptococcus faecalis which are clinically isolated and identified by our group. The culture broth is Brain Heart Infusion (BHI). Much effort has been paid on making suitable cell. From the view of sterilization and repeatedly use, the cells are made by glass. The turbidity is measured by pair of CdS-LED (red) elements. The impedance is measured by means of previously proposed four-electrode method. The electrodes are gold and stainless-steel wires (0.7mmφ) which are verified to give no difference in measuring impedance. The recorded impedance decrease is good in its reproducibility comparing it to that of twoelectrode method. The rate of the change of these two quantities are computed from the recorded data yielding reproducible curves against time (denoted as ”rate pattern”). The rate patterns for impedance and turbidity give inherent ones for examined 6 bacteria. Some numerical values, peak value, half width, and so on, of rate patterns are measured from the curves. Some ratios and absolute values of parameters are selected as trial characteristic parameters. Bacteria are classified by each characteristic parameters. 5 characteristic parameters are needed for identifying 6 becteria. The rate patterns seem to be useful in screening or identification of clinical bacteria.
Subjects
電気電子工学 ( Other)
Languages jpn
Resource Type departmental bulletin paper
Publishers 山口大学工学部
Date Issued 1980
File Version Version of Record
Access Rights open access
Relations
[ISSN]0372-7661
[NCID]AN00244228
Schools 工学部