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Yamashita Hiroshi

Affiliate Master Yamaguchi University

The free radical scavenger edaravone protects hair cells against aminoglycoside toxicity

The bulletin of the Yamaguchi Medical School Volume 55 Issue 1-2 Page 15-20
published_at 2008
A050055000103.pdf
[fulltext] 1.84 MB
Title
The free radical scavenger edaravone protects hair cells against aminoglycoside toxicity
Creators Arai Tsuguyuki
Creators Sugahara Kazuma
Creators Mikuriya Takefumi
Creators Miyauchi Yuji
Creators Hirose Yoshinobu
Creators Hashimoto Makoto
Creators Shimogori Hiroaki
Creators Yamashita Hiroshi
Source Identifiers
Creator Keywords
hair cell aminoglycoside hydroxy radical Edaravone ototoxicity
Reactive oxygen species are involved in inner ear hair cell death induced by aminoglycosides. Therefore, inhibition of free radicals can prevent hair cell death. The antioxidant, edaravone has been used clinically in Japan. In this study, we evaluated whether edaravone can protect hair cells against aminoglycoside-induced cytotoxicity in cultured mouse utricles. Utricles from CBA/N mice were cultured in medium containing neomycin alone (1 mM), or neomycin plus edaravone. Hair cells cultured with neomycin alone were damaged after 24 hours. Survival of hair cells cultured with neomycin plus edaravone (30 or 100 μM) was significantly greater than that of hair cells cultured with neomycin alone. Immunohistochemistry showed the production of 4-hydroxy-2-nonenal in utricles cultured with neomycin but not in utricles cultured with neomycin plus edaravone (100 μM). These results suggest that the inhibibion of reactive oxygen species production by edaravone protects hair cells. In addition, high concentrations of edaravone did no show cytotoxicity.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University School of Medicine
Date Issued 2008
File Version Version of Record
Access Rights open access
Relations
[isVersionOf] [URI]http://ds.cc.yamaguchi-u.ac.jp/~bulletin/index.html
Schools 医学部