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Sasaki Kohsuke

Affiliate Master Yamaguchi University

Cell Cycle Dependent Con-A binding-Flow Cytometric Analysis-

The bulletin of the Yamaguchi Medical School Volume 31 Issue 1 Page 1-5
published_at 1984-12
A020031000101.pdf
[fulltext] 1.04 MB
Title
Cell Cycle Dependent Con-A binding-Flow Cytometric Analysis-
Creators Sasaki Kohsuke
Creators Takahashi Manabu
Creators Ogino Tetsuro
Creators Murakami Tomoyuki
Creators Kawasaki Shoji
Creators Nagaoka Sakae
Source Identifiers
Creator Keywords
Con A Flow cytometry Cell Cycle DNA
Changes in lectin binding activity occuring the cell cycle were determined flow cytometrically in exponentaiiy growing YU-101 cells by a technique of gated analysis based on cellular DNA content after double staining of the cells with fluorescein isothiocyanate (FITC) and propidium iodide (PI). Fluorescence intensity from FITC conjugated concanavalin A (Con A) increased during the cell cycle and reached maximum in G2+M phase. Binding of the lectins was reduced by half in G1 phase after cell division. Binding activity of Con A and in YU-101 cells was linked closely with cell cycle. It is surrested that the nuclear changes are associated with alterations of the cellular membrane and that molecular communications between the cell membrane and the cell nucleus control periodic cell cycle. The methodology developed in these studies gives reliable data since a procedure for cell synchronization which brings out some effects on the normal function of the cell cycle is unnecessary.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1984-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部