走査電子顕微鏡標本調整法の研究 特に微生物領域における研究 第V編 特殊処理法について 第1報 光学顕微鏡および走査電子顕微鏡による同一視野観察のための処理法

Studies on the Specimen Preparation Method for Scanning Electron Microscopy PartV. Special Treatment for Specimen Peparation. 1. Preparation on the Specimen for Observing of the Same and Fixed Opical Field by Light Microscope and Scannig Electron …

Creators Yoshii Zensaku

Creators Konishi Hisanori

Creators Tanaka Shuhei

Creators Hiruki Takeo

Creators Takamura Akira

Creators Kazuhisa Takao

Creators Sugiyama Junichi

Creators Ejima Toshihiro

Creators Ito Takao

Creators Kamata Mizuho　

In order to observe the same and fixed opical field in the specimen by both light microscope (LM) and scanning electron microscope (SEM) , some pretreatments were contrived for specimen preparation. That is the marking technique on the glass slide piece by using object marker was adopted. Moreover a system of technical procedures, such as light microscopy and photography by using the dry or water immersion system of object lens, searhing and triming of the same and fixed opical field on the SEM-screen by collating of both images with printed LM-graph obtaining of observations after printing of both images, was established. The usefullness of this system was shown to be practical by using some samples. Therefore, the simplicity and certainty of this system is considered to be worthy of utility.

[ISSN]0513-1731

[NCID]AN00243156