n Experimental Research on the Relation between the Expression of Proliferation Associated Antigens and the Transition from Quiescent Phase to S-phase of Cell Cycle
山口医学 Volume 44 Issue 2
Page 105-111
published_at 1995-04
Title
増殖関連抗原の発言とS期移行との関連性に関する実験的研究
n Experimental Research on the Relation between the Expression of Proliferation Associated Antigens and the Transition from Quiescent Phase to S-phase of Cell Cycle
Source Identifiers
Creator Keywords
PCNA
BrdU
Ki-67
PHA刺激リンパ球
細胞周期
PCNA is an auxiliary protein of DNA polymerase d, and synthesized during late Gl to S phases in proliferating cells. The cells expressing Ki-67 reactive antigen, another proliferation associated antigen are supposed to be cycling cells. Correlations between positive rate of these antigens and S phase ratio are often discussed, but the relations between expression of these antigens and the transition to S phase are still controversial. Quiescent cells need to express PCNA to enter the proliferating phase, but all cell expressing PCNA do not necessarily enter the S phase. It is true of Ki-67 reactive antigen positive cells, too. In this study, the order of the expression of PCNA and Ki-67 weather arnigen were observed during the transition from quiescent phase to proliferating phase of cell cycle using normal human lymphocytes stimulated by mitogenic agent of PHA (bacto-phytohemagglutinin M). As a result, at 24 hours after PHA stimulation, the PCNA positive rate began to inerease. After another 12 hours, Ki-67 reactive antigen positive rate, and at 48hours after stimulation, BrdU labeling index also began to increase. The correlations between the positive rates of PCNA and Ki-67 reactive antigen, PCNA and BrdU in human tumor tissues were also examined using 15 clinical cases. The result shows that all have significant positive correlation (PCNA:BrdU, r=0.73, p<0.05, Ki-67 reactive antigen:BrdU, r=0.82, p<0.01, PCNA:Ki-67 reactive antigen, r=0.92, p<0.01). There are some differences among the correlations, which may be caused by the order of expression of these proliferation associated antigens.
Languages
jpn
Resource Type
journal article
Publishers
山口大学医学会
Date Issued
1995-04
File Version
Not Applicable (or Unknown)
Access Rights
metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools
医学部