Monoclonal antibody against human colonoc adenoma

ヒト大腸線腫に対するモノクローナル抗体の作製

Monoclonal antibody against human colonoc adenoma

Creators Miyahara Taeko

Creators Fujikura Yoshihisa

Creators Harima Takashi

Creators Yasutake Ryujiro

Creators Matsuura Shinjiro

Creators Yoshimura Syunji

Creators Fukumoto Tetsuo

Creators Takemoto Tadayoshi

We have generated weo monoclonal antibodies (UB-21, UB-22), which were produced by hybridoma cells, fusing mouse myeloma cells (NS-1) with the spleem cells of a BALB/c mouse that has been immunized with the suspended human colonic adenomas. Using an indirect immunofluorescence method, the presence of the anatigen against UB-21 and UB-22 was detected : it was detected in the normal human liver. UB-21 was detected in the large and small intestine of monkey, but UB-21 and UB-22 were negative with the intestine of the rat, rabbit raccoon, and badger. These findings suggested that these monoclonal antibodies (UB-21, UB-21) were specific for human and monkey's intestinal component and might be useful for analyzing normal and milignant human intesine.

[ISSN]0513-1731

[NCID]AN00243156