NO2- and NO3- Measurements for Detection of Escherichia coli in Blood Samples
The bulletin of the Yamaguchi Medical School Volume 46 Issue 3-4
Page 93-97
published_at 1999-12
Title
NO2- and NO3- Measurements for Detection of Escherichia coli in Blood Samples
Creators
Tsuruta R.
Creators
Matsuno Y.
Creators
Mizuno H.
Creators
Sadamitsu D.
Creators
Maekawa T.
Source Identifiers
Creator Keywords
Escherichia coli
capillary zone electrophoresis
bacteremia
CO2 production
Escherichia (E..) coli, a common causative misroorganism in bacteremia, is a member of facultatively anaerobic bancilli that are able to reduce NO3- to NO2-. To determine whether NO2- production or NO3- reduction occurs during the growth of E.. Coli in blood cultures, NO2- and NO3- were directly and simultaneously neasured by capillary zone electrophoresis. The BACTEC 9240 (Becton Dickinson Diagostic Instrument Systems, USA) was used to culture E. coli cells in blood samples. The NO2- concentration in the medium (6.0±2.6 μM at 0 h) significantly increased at 8 h (p < 0.0001) and lowered to 65.0±37.6 μM at 10h. The peak of NO2- production (9.2±o.7h) appeared earlier than the CO2 production (11.1±0.9h). Theree is no correlation between the peak time of NO2- production and the CO2 detection time (p > 0.05). These data give us an essential potentiality to use the NO2- and NO3- monitoring system for detecting the presence of E. coli cells.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1999-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部