Fas and Fas-ligand Expression in Hepatocytes during Regeneration after Partial Hepatectomy in Rats
The bulletin of the Yamaguchi Medical School Volume 45 Issue 1-4
Page 29-37
published_at 1998-12
Title
Fas and Fas-ligand Expression in Hepatocytes during Regeneration after Partial Hepatectomy in Rats
Creators
Ogura Yutaka
Source Identifiers
Creator Keywords
Fas
Fas-ligand
Apoptosis
Hepatocyte
Liver regeneration
The regenerative response after partial hepatectomy stops suddenly when liver mass has been restored. In this resulation, arrest of hepatocyte proliferation in addition to elimination of excess hepatocytes would appear to be necessary. To determine whether excess hepatocytes are eliminated by Fas-mediated apoptosis, we examined expression of Has and Fas-ligand in hepatocytes using immunohistochemical staining ofter 70% partial hepatectomy. In addition, apoptotic hepatocytes were identified using in situ DNA end-labeling. Fas-positive, Fas-ligand-positive, and apoptotic hepatocytes ware detected only rarely in the uninjured liver. Fas-positive hepatocytes increased somewhat on day 3 follwing hepatectomy and signficantly increased somewhat on day 4, markedly on day 6, and then desreased on day 14. According to these findings, apoptosis began to occur in hepatocytes 1 day after initial expression of Fas and its ligand. These apoptotic changes may be explained by the consideration that some abruptly regenerated hepatocytes of Fas and its ligand may indicate paracrine or autocrine mechanisms of cell elimination executed by the hepatocytes themselves.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1998-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部