Purification Method of Proteins by Preparative Gel Electrophoresis. Crystallisation of α-amylases.
The bulletin of the Yamaguchi Medical School Volume 23 Issue 3-4
Page 165-183
published_at 1976-12
Title
Purification Method of Proteins by Preparative Gel Electrophoresis. Crystallisation of α-amylases.
Creators
Takeo Kazusuke
Creators
Suzuno Ryosuke
Creators
Fujimoto Masanori
Creators
Kuwahara Akira
Creators
Ogata Hatanori
Creators
Nakamura Shojiro
Source Identifiers
Taka-amylase A from Aspergillus orizae and huma α-amylase were crystallized in a three-step procedure: (1) Crude extract o fTaka-diastase (Sankyo) or saliva supernatant was applied to a gel slab type apparatus of preparative electrophoresis. The active fractions were extracted by crushing the gel slices with a buffer solution.(2) The soluble gel components, which were extracted from the gel slices together with protein fractions, were removed by electrophoresis with a discontinuous buffer system containing sucrose gradient (purification electrophoresis).(3) After acetone fractionation, crystalline amylases were obtained. The overall recovery rates of Taka-amylase A and human salivary amylase were 51% and 53%, respectively. Further usage of preparative gel electrophoresis and purification electrophoresis to obtaining pure proteins was examined.
Languages
eng
Resource Type
departmental bulletin paper
Publishers
Yamaguchi University Graduate School of Medicine
Date Issued
1976-12
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools
医学部