Age-related changes in activation and inactivation kinetics of the Na channels in cultured heart muscle from chick embryo
Memoirs of the Faculty of Engineering, Yamaguchi University Volume 41 Issue 2
Page 369-377
published_at 1991-03
Title
ヒヨコ胚培養心筋細胞でのNaチャネルの活性・不活性化パラメータの加令による変化
Age-related changes in activation and inactivation kinetics of the Na channels in cultured heart muscle from chick embryo
Creators
Ebina Yoshio
Creators
Sada Hideaki
Creators
Toshitani Hideo
Creators
Nakajima Kazuki
Creators
Ban Takashi
Source Identifiers
To investigate age-related changes in Na^+ channel kinetics, ventricular cells obtained from chick embryos of various developmental stages were voltage-clamped with patch pipettes, and the Na^+ current (I_<Na>) together with the membrane potential (V_m) were recorded (16℃). The data stored in VTR as PCM signal was A/D converted and analyzed in terms of Hodgkin-Huxley model. I_<Na> underwent the activation/inactivation sequence in response to change in V_m. While the activation kinetics little changed throughout embryogenesis, the inactivation kinetics was altered with embryonic development. Yet, the density of Na^+ channel per unit membrane area also was drastically increased with development. We discuss the present voltage-clamp data in comparison with those obtained from different tissues and/or animal species.
Languages
jpn
Resource Type
departmental bulletin paper
Publishers
山口大学工学部
Date Issued
1991-03
File Version
Version of Record
Access Rights
open access
Relations
[ISSN]0372-7661
[NCID]AN00244228
Schools
工学部