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An Ultramicro-Colorimetric Method for the Determination of Plasma Glucose with O-Aminobiphenyl, Circumventing Deproteinzation

The bulletin of the Yamaguchi Medical School Volume 8 Issue 3 Page 209-214
published_at 1961-09
A020008000302.pdf
[fulltext] 370 KB
Title
An Ultramicro-Colorimetric Method for the Determination of Plasma Glucose with O-Aminobiphenyl, Circumventing Deproteinzation
Creators Shibata Susumu
Source Identifiers
A new direct method using o-aminobiphenyl reagent for the determination of glucose in serum or plasma has been described. To 0.05 ml of serum are added 0.6 ml of saturated boric acid solution and 4.2 ml (sometimes 4.4 ml) of 0.5g/dl oaminobiphenyl dissolved in grlacial acetic.acid The mixture is heated in boiling water bath for 30 minutes. The resultant bluish green color is measured for absornace in a photoelectric colorimeter at 650mμ. No deproteinization is required. The values thus obtained compare quite well with those obtained with Somogyi-Nelson's method.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1961-09
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部