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The Periodic Loss of Metabolically Unstable DNA from Newly Replicated DNA of Mouse L Cells

The bulletin of the Yamaguchi Medical School Volume 27 Issue 3-4 Page 117-124
published_at 1980-12
A020027000305.pdf
[fulltext] 486 KB
Title
The Periodic Loss of Metabolically Unstable DNA from Newly Replicated DNA of Mouse L Cells
Creators Masuda Michiyoshi
Creators Yamaoka Hideki
Creators Isiglo Kimio
Creators Matsumoto Takafumi
Creators Takahashi Manabu
Source Identifiers
Creator Keywords
^3H-thymidine incorporation unstable DNA hydroxyurea
The kinetic constants for human very low density lipoproteins (VLDL) of membrane-supported lipoprotein lipase (LPL) of the rat were studied. The VLDL substrate (Sf20-400) was obtained from the plasma of healthy human subjects. with normolipidemia and with normal patterns of VLDL apoprotein peptides in isoelectric focusing electrophoretogram. Triglycerides of human VLDL were hydrolyzed by LPL on the vasclar surface of the isolated rat heart. The calculated maximal reaction velocity (Vmax) and the apparent Michaelis constant (Km) were 0.037±0.006μmol min^-1ml^-1 and 0.060±0.004mM (Mean±S.D.), respectively. These values were very close to the corresponding values obtained using rat VLDL as a substrate. This system provides a means to study possible kinetic abnormalities of the triglyceride-rich lipoproteins on the vascular surface of the heart of hyperlipidemic patients.
Subjects
医学 ( Other)
Languages eng
Resource Type departmental bulletin paper
Publishers Yamaguchi University Graduate School of Medicine
Date Issued 1980-12
File Version Version of Record
Access Rights open access
Relations
[ISSN]0513-1812
[NCID]AA00594272
Schools 医学部