TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice

TNF-α and IL-8 Release by Sinusoidai Endothelial Cells during Hypoxia-reoxygenation in Rats with Obstructive Jaundice

Creators Hayashi Daisuke

The release of of cytokines by sinusoidal endothelial cells (SECs) during hypoxia reoxygenation was studied in vitro in male Wistar rats with obstructive jaundice to investigate the kinetics of tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) release by SECs during hypoxia-reoxygenation. The rats were divided into two groups according to the procedure perfomed. An obstructive jaundice group (Group OJ) underwent bile duct ligation and a control group (Group C) underwent a sham-operation. SECs were isolated by collagenase digestion and centrifugal elutriation. After exposing the cells to hypoxia in 95% oxygen. The levels of TNF-α and IL-8 were significantry decreased in group OJ compared with those in Group C, indicationing that obstructive jaundice induced functional damage to SECs with decreased cytokine production during hypoxia-reoxygenation.

[ISSN]0513-1812

[NCID]AA00594272