Detection of chimerism following rat limb allograft

Chimerism following rat limb allograft was assessed by a technique of molecular biology. Genetically inbred LEW rats with a defined major histocompatibility complex were used and their vascularized whole-limb transplantations were performed between pairs of rats sex-mismatched. A semi-quantitative polymerase chain reaction for Y-chromosome following the sex-mismatched whole-limb transplantations enabled us to identify the donor-derived (male to female transplantation) or the recipient-derived (female to male transplantation) cells. The donor cells were detected in recipient lymphoid tissues at 48 weeks after the transplantation and microchimerism was concluded in recipient tissues. The donor cells in the grafted bone, skin and muscle were gradually repopulated with the recipient-originated cells. Because limb allograft tissues include the bone marrow, a source of cell-supply, systemic microchimerism is concluded in recipient tissues and limb allograft tissues are immunologically useful. Since bone is a unique for generating the organ with mesenchymal cells in the process of its reconstruction, an interchange of cells from recipient progresses in the early stage. This study indicates that organs of muscle and skin which maintain their constancy by self-regeneration, also show the potentiality of graft chimerism.

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キメリズム

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本文データは山口大学医学会の許諾に基づきCiNiiから複製したものである