Studies on the Specimen Preparation Methods for Scanning. Electron Microscoy PartIII. Drying Method of Microbial and Free Cell Materials 2. A contrivance in Critical Point Drying : Utilization of the Small Envelope of Filter Paper.

A new idea was introduced into the specimen preparation method of microbial or free cell materials　for scanning electron microscopy. Utilization of the small envelope of filter paper, including glass piece of aluminum foil, to the critical point drying method was very useful for simplification of technical procedures and effective drying of samples. Deｈｙｄration by passing the materials through graded concentrations of acetone was emphasized. This simpler procedure was possible to diminish the double treatments, passing of ethanol dehydration and replacing of amyl acetate. The direct replacement of samples from acetons to liquid carbon dioxide in the chamber was possible and… more convenient. The inside wall of small envelope is usually abundant with attached sampels, so that a piece of envelope is possible to utilize for specium preparation in addition to the included glass or aluminum foil.