Contents Menu

Implication of activation of intraperitoneal macrophages with bio-degradable microspheres

Cell biology international reports Volume 13 Issue 6 Page 547-553
published_at 1989-06
Title
Implication of activation of intraperitoneal macrophages with bio-degradable microspheres
Creators Okawa T.
Creators Kagabu Satoshi
Creators Ishida T.
Creators Kawata M.
Creators Kagabu S.
Creators Mamba K.
Creators Makita T.
To estimate the possibility that biological degradable starch microspheres (DMS) activate abdominal or intraperitoneal macrophages (IMP), two sizes of DMS (Spherex, Pharmacia, Sweden) were injected into the peritoneum of the ICR mice of 4 to 8 weeks of age. Three days after the injection, peritoneal fluid was collected and incubated for one hour at 37℃ under 5% CO_2. The cells which adhered to the petri dish were IMP, to which DMS was added for 18 hrs. The cultured IMP were observed by scanning electron microscope (SEM) and the ratio of the active type to the total number of IMP was counted as an index of the effect of DMS to IMP. The activation effect of DMS on the incubated IMP was significant in the group which was cultured with 2μm DMS after the 45μm DMS injection. That indicated the possible DMS function as a potential IMP activating factor (MAF).
Languages eng
Resource Type journal article
Publishers Elsevier
Date Issued 1989-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0309-1651
[NCID]AA00600025
info:doi/10.1016/0309-1651(89)90101-X
[isVersionOf] [URI]http://www.sciencedirect.com/science/journal/03091651
Schools 農学部