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An experimental study on bone allograft in rats : using immunohistochemistry and radioimmunoassay

山口医学 Volume 39 Issue 3 Page 421-431
published_at 1990-06
Title
ラット同種骨移植に関する実験的研究 : 移植片ならびに宿主における免疫反応の免疫組織化学的解析を中心として
An experimental study on bone allograft in rats : using immunohistochemistry and radioimmunoassay
Creators Shigetomi Mitsunori
Source Identifiers
The purpose of this study was to investigate the rejection mechanism of the allografted bone tissue through the change of the location and intensity of the major histocompatibility complex (MHC) antigen expression on the osteocyte and the changes in the alloantibody levels using immunohistochemistry and tadioimmunoassay. The following conclusions were obtained through this study. (1) Some osteocytes wxpress MHC Class I antigen in the normal rat bone tissue. The expression of MHC Class I antigen on osteocytes showed diversity. However, the expression of MHC Class I antigens on osteocytes ofallografted bone tissue uncreased with time after bone allograft transplantation. (2) Three weeks after allograft transplantation, a severe decrease of osteocytes in allografted bone tissue was observed. Almost all the remaining osteocytes expressed MHC Class I antigen strongly in the allografted bone tissue in DA to F334 strain combination. (3) F334 weeks recipients of DA rat bone grafts developed high titers of allonatibody anti-Class I. This titer increased from 2 weeks after transplantation and it kept still high levels even 14 weeks after transplantation. (4) MHC Class I antigens might play an important role in bone allograft rejection as has been observed in other transplanted organs in the rat.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1990-06
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部