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Microassay for Proteins on Nitrocellulose Filter Using Protein Dye-Staining Procedure

山口医学 Volume 34 Issue 5 Page 373-378
published_at 1985-10
Title
ニトロセルロース膜を用いたタンパク微量測定法
Microassay for Proteins on Nitrocellulose Filter Using Protein Dye-Staining Procedure
Creators Nakamura Kazuyuki
Creators Tanaka Tatehiko
Creators Fujimoto Masanori
Creators Kuwahara Akira
Creators Suzuno Ryousuke
Creators Suzuki Isao
Creators Takeo Kazusuke
Source Identifiers
A simple and rapid microassay for proteins utilizing the protein dye-staining procedure with a nitrocellulose (NC) filter is described. Proteins were directly bound to an NC filter using the ”BIO DOT” microfiltration apparatus to ensure their uniformity. The proteins were then stained with a dye, and the optical density of the stained protein spots was directly measured by a densitometer. A good linearity between the optical density and the amount of protein was obtained in the range 0.05-10μg. A larger number of samples (up to 96 samples) could be assayed within 1.5 h simultaneously. Furthermore, contaminating chemicals, such as amino acids, sugars, reducing agents, chelating agents, deoxyribonucleic acid, nucleotides, or tris (hydroxymethyl) aminomethane, did not interfere with the assay. The usefulness of the assay for the quantitation of a small amount of proteins in body fluids is discussed.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1985-10
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部