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Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.)

Genes & genetic systems Volume 81 Issue 4 Page 255-263
published_at 2006
2009010009.pdf
[fulltext] 1.1 MB
Title
Complete assignment of structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of the shallot (Allium cepa L.)
Creators Masuzaki Shin-ichi
Creators Shigyo Masayoshi
Creators Yamauchi Naoki
Creator Keywords
alien chromosome addition line Allium cepa Allium fistulosum chromosomal location flavonoid biosynthetic gene
We analyzed Japanese bunching onion (Allium fistulosum L.) - shallot (Allium cepa L. Aggregatum group) alien chromosome addition lines in order to assign the genes involved in the flavonoid biosynthesis pathway to chromosomes of the shallot. Two complete sets of alien monosomic additions (2n = 2x + 1 = 17) were used for determining the chromosomal locations of several partial sequences of candidate genes, CHS, CHI, F3H, DFR, and ANS via analyses of PCR-based markers. The results of DNA marker analyses showed that the CHS-A, CHS-B, CHI, F3H, DFR, and ANS genes should be assigned to chromosomes 2A, 4A, 3A, 3A, 7A, and 4A, respectively. HPLC analyses of 14 A. fistulosum - shallot multiple alien additions (2n = 2x + 2 - 2x + 7 = 18 - 23) were conducted to identify the anthocyanin compounds produced in the scaly leaves. A direct comparison between the genomic constitution and the anthocyanin compositions of the multiple additions revealed that a 3GT gene for glucosylation of anthocyanidin was located on 4A. Thus, we were able to assign all structural genes involved in flavonoid biosynthesis influencing bulb color to individual chromosomes of A. cepa.
Languages eng
Resource Type journal article
Publishers 日本遺伝学会
Date Issued 2006
File Version Version of Record
Access Rights open access
Relations
[ISSN]1341-7568
[NCID]AA11077421
https://doi.org/10.1266/ggs.81.255
[isVersionOf] [URI]http://www.jstage.jst.go.jp/browse/ggs/_vols
Schools 農学部