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Studies on the Biospecific Molecular Interactions by Means of Affinity Electrophoresis

山口医学 Volume 31 Issue 5 Page 417-430
published_at 1982-10
Title
Affinity Electrophoresisによる生物特異相互反応の研究
Studies on the Biospecific Molecular Interactions by Means of Affinity Electrophoresis
Creators Takeo Kazusuke
Source Identifiers
The principle of the affinity electrophoresis was summarized. Three affinity equations, the original, the inhibition, and the general affinity equations, were drived from the affinity theory. According to those equations, dissociation constants of various enzymes for high and low molecular substrates, lectins for oligo- and polysaccharides, anti-dextran myeloma proteins for dextran and isomaltose oligosaccharides, and anti-DNP-antibodies for DNP-polyacrylamide copolymer were determined. For highly heterogeneous protein mixtures, the two-dimensional affinity electrophoresis showed an excellent resolution. Thus, rabbit anti-DNP-antibodies were separated into over 200 spots on one affinity gel. Dissociation constants for every IgG spot were directly calculated from the affinity pattern. Effects of temperature and pH on the affinity electrophoresis were discussed.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1982-10
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部