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Studies on a Strain of Bacillus brevis Isolated from Soil : Part II. Purification of Antibiotics and their Characteristics

山口医学 Volume 32 Issue 2 Page 161-172
published_at 1983-04
Title
土壌由来のBacillus brevis1菌株の抗生物質学的研究 : (第2報) 抗生物質の単離とその諸性状
Studies on a Strain of Bacillus brevis Isolated from Soil : Part II. Purification of Antibiotics and their Characteristics
Creators Motoki Yoshinobu
Source Identifiers
Two kinds of antibiotics were harvested from a strain recently isolated. The supernatant of the culture at 28℃ showed a broad spectrum against Gram-positive and -negative bacteria. However, the supernatant of the culture at 37℃ showed growth inhibition only against Gram-positive bacteria. These substances were separated and purified through the successive steps involving column chromatography using Amberlite CG-50 and Sephadex G-25. These two antibiotics were identified as Gramicidin S and Polymyxin A. Furthermore, it was shown that these two antibiotics were simultaneously produced at 28℃. Gramicidin S was also produced at temperatures up to 40℃, however, Polymyxin A was not above 35℃. Two mutants lacking of polymyxin producing ability were obtained from the strain by acriflavin treatment.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1983-04
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部