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Some Observations on Peroxydase Reaction of Leukocytes

山口医学 Volume 20 Issue 1 Page 11-18
published_at 1971-03
Title
白血球ペルオキシダーゼ反応の検討
Some Observations on Peroxydase Reaction of Leukocytes
Creators Nakano Tamotsu
Source Identifiers
The improved method of peroxydase reaction for human leukocyte analysis was studied comparing with the recommended clinical laboratory methods. This new method ”Benzidine-Zinc Method” differs from others in the pretreatment with ZnSO_4 solution, although it principally depends on the oxydation of benzidine by the peroxyde-peroxydase system. The main procedures of this method are following in order, fixation with 10% formalin-alcohol, Giemsa staining, 1% ZnSO_4 treatment and the benzidine-peroxide reaction lastly. The specificity of this reaction to leukocyte peroxydase was confirmed by careful comparison with the results of Sato-Sekitani's and Mc Junkin's methods. It is found that this method is more excellent at three points. First, the reaction products in blood cells are observable very clearly, that is intense blue in neutrophils, yellowish brown in acidophils and faint blue in monocytes. Secondly, identification of blood cell types is very easy according to per-staining wiith Giemsa solution. Thirdly , red corpuscles and blood platatelets are well preserved, and the pseudo-peroxydase reaction in red cells is negrigible. This method was applied to some Patients with leukemia and others, and the results obtained were discussed. Thus this ”Benzidine-Zinc Method” of peroxydase reaction seems to be reliable and excellent for clinical laboratory use.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1971-03
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部