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Identification of Abnormal Hemoglobins IV. Polyacrylamide Gel Electrophoresis of the Globin Chains

山口医学 Volume 29 Issue 3 Page 261-268
published_at 1980-09
Title
異常血色素の同定法 IV グロビン鎖のポリアクリルアミドゲル電気泳動
Identification of Abnormal Hemoglobins IV. Polyacrylamide Gel Electrophoresis of the Globin Chains
Creators Fujisawa Keiko
Creators Muroya Satomi
Creators Minooka Hiromi
Creators Ohba Yuzo
Source Identifiers
Polyacrylamide gel electrophoresis of the globin chains using two buffer systems, tris-glycine (pH9.8) and β-alanne acetate (pH4.6) in concentrated urea, was studied by column and slab methods. Concentration of urea was reduced from 8 M to 6.4 M for convenience without change in resolving power. In order to save time, total acrylamide concentration of 4.9g/dl was preferred to 7.3g/dl. The pH 9.8 buffer was quite suitable for the separation of the α,β and σ chains, while the pH 4.6 buffer was particularly useful for the separation of the γ from the βchain and of the N-acetyland γ chain from the unmodified one…
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1980-09
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部