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Identification of Abnormal Hemoglobins : V. Detection and Identification of Unstable Hemoglobins

山口医学 Volume 32 Issue 1 Page 87-104
published_at 1983-02
Title
異常血色素の同定法 : V. 不安定ヘモグロビンの検出と同定
Identification of Abnormal Hemoglobins : V. Detection and Identification of Unstable Hemoglobins
Creators Ohba Yuzo
Creators Hattori Yukio
Creators Yamamoto Kiyomi
Creators Matsuoka Miyoko
Creators Miyaji Takaoki
Source Identifiers
An over-all discussion on authors' experience in the detection and identification of 15 unstable hemoglobins causing hemolytic disease and 6 slightly unstable, clinically harmless variants is presented. The diagnostic efficiency of routine screening procedures including erythrocyte oxygen equilibrium curve, glycerol lysis time, starch gel electrophoresis of hemolysate, levels of Hb A_2 and Hb F, qualitative heat precipitation and isopropanol tests was compared with that ot quantitative proccdure of heat denaturation, PCMB (p-chloromercuribenzoic acid) precipitation, mechanical shaking and Sephadex G-75 gel filtration. Urea polyacrylamide gel electrophoresis of the PCMB precipitate was superior to the other methods as a method of detection of unstable hemoglobins and identification of chain anomaly. The PCMB precipitate proved to be a satisfactory starting material for the purification of the abnormal globin by urea CM-cellulose column chromatography. A systematic approarch to the laboratory diagnosis of unstable hemoglobin hemolytic disease was proposed.
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1983-02
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部