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Study of the Interaction Between NAD- and NADP- Dependent Dehydrogenases and Immobilzed Adenine Nucleotidesvby Means of Affinity Electrophoresis I. Synthesis of Water-Soluble 2'-AMP- and 5' -AMP- Acrylamide Copolymers as Affinity Ligand Used for Affinity

山口医学 Volume 30 Issue 3 Page 251-257
published_at 1981-09
Title
Affinityelectrophoresisによる固定化アデニンヌクレオチド誘導体とNAD-及びNADP-依存性脱水素酵素との特異結合反応の解析 I. 水溶性高分子リガンドである2'-AMP及び5'-AMP-アクリルアミド共重合体の合成
Study of the Interaction Between NAD- and NADP- Dependent Dehydrogenases and Immobilzed Adenine Nucleotidesvby Means of Affinity Electrophoresis I. Synthesis of Water-Soluble 2'-AMP- and 5' -AMP- Acrylamide Copolymers as Affinity Ligand Used for Affinity
Creators Nakamura Kazuyuki
Source Identifiers
Water-soluble adenosine 2'-monophosphate (2'-AMP)-and adenosine 5' -monophosphate (5'-AMP) -acrylamide copolymers were synthesized as new affinity ligands used for affinity electrophoresis to determine the dissociation constants of NAD- and NADP- dependent dehydrogenases for 2'-AMP and 5'-AMP. Reaction of 5'-AMPwith bromine at pH 4.0 yielded 8-bromo-5'-AMP and following reaction with allyamine at pH 9.0 yielded 8-allyl-5'-AMP with acrylamide, a water-soluble high molecular weight 5'-AMP-acrylamide copolymer can be obtained. 2'-AMP-acrylamide copolymer was synthesized by the same procedure as 5'-AMP-acrylamide copolymer synthesis…
Subjects
医学 ( Other)
Languages jpn
Resource Type journal article
Publishers 山口大学医学会
Date Issued 1981-09
File Version Not Applicable (or Unknown)
Access Rights metadata only access
Relations
[ISSN]0513-1731
[NCID]AN00243156
Schools 医学部